New technology in experimental neurobiology: neuronal networks coupled with multielectrode array

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Abstract

Multielectrode arraуs (MEA) as the recording and stimulating system for self-organizing, functionally heterogeneous neuronal network formed by developing CNS cells in vitro is a new, unique technology for investigation of mechanisms of pathological neurodestructive processes and for searching the means of their pharmacological corrections. The principal advantages of the MEA are the precise noninvasive long-term network stimulation and measurement of the electric signals, pharmacological testing and drug screening, optical structural and functional imaging of metabolic ionic current into neurons and glial cells using confocal laser scanning microscopy. The brain cells and tissues coupled in vitro with MEA enables neuroprotective and/or neurotoxic drug testing in different models of the central nervous system pathological states. Data-intensive part, good reproducibility and quantitative assessment capability make it possible to relate the neuron networks cultured to the MEA with biosensors, allowing to perform effective pharmacological screening in vitro for the models of ischemia, trauma, epilepsy, Alzheimer’s disease, etc.

 

About the authors

I. V. Mukhina

Nizhny Novgorod State Medical Academy

Email: haspekleon@mail.ru
Russian Federation, Nizhny Novgorod

Leonid G. Khaspekov

Research Center of Neurology

Author for correspondence.
Email: khaspekleon@mail.ru
Russian Federation, Moscow

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