Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental Neurology2075-54732409-2533Research Center of Neurology1910.17816/psaic19UnknownTransplantation of neuronal precursors derived from induced pluripotent stem cells into the striatum of rats with the toxin-induced model of Huntington’s diseaseStavrovskayaAlla V.alla_stav@mail.ruYamshchikovaNina G.alla_stav@mail.ruOl'shanskyАrtyem S.alla_stav@mail.ruKonovalovaEugeniya V.alla_stav@mail.ruIllarioshkinSergey N.<p>D. Sci. (Med.), Prof., Corr. Member of the Russian Academy of Sciences, Deputy Director, Head, Department for brain research</p>alla_stav@mail.ruhttps://orcid.org/0000-0002-2704-6282Research Center of Neurology02122016104394430012017Copyright © 2016, Stavrovskaya A.V., Yamshchikova N.G., Ol’shanskiy A.S., Konovalova E.V., Illarioshkin S.N.2016<p><strong>Introduction.</strong>Huntingtons disease (HD) is a severe neurodegenerativedisorder characterized by choreic hyperkinesis, cognitivedecline, behavioral disorders, and progressive neuronal death,mostly in the striatum. Since HD is a fatal disorder, searchingfor efficient treatment methods, including those based on cell replacementtherapy, is quite relevant. The experimental models ofHD are used increasingly often.<br />The<strong>objective</strong>of the study was to assess effectiveness and safetyof transplantation of neuronal precursors differentiated from inducedpluripotent stem cells (iPSCs) from a healthy donor into thestriatum of rats with 3-NPA-induced HD model.<br /><strong>Materials and methods</strong>. We studied the influence of neurotransplantationon the behavioral effects in rats with HD model inducedby intrastriatal injection of 3-nitropropiotic acid (3-NPA). In the study group of animals (n=11), human neuronal precursorsderived from iPSCs of a healthy volunteer were transplantedinto the caudate nuclei (5105 per 5 l of normal saline solutionbilaterally); the control group of animals (n=10) received normalsaline solution. The animals were tested using the Any-maze videotracking system; the parameters of the open-field test and the conditionedavoidance response test were evaluated.<br /><strong>Results.</strong>An analysis of behavioral effects after transplantationdemonstrated that introduction of neuronal iPSC derivatives intothe caudate nuclei of rats with induced HD model was accompaniedby recovery of motor activity of the animals (horizontaland vertical), as opposed to the control group. It was found duringtesting the reproducibility of the conditioned avoidance responsesthat the conditioned avoidance responses in control animalswere weakened, whereas intrastriatal transplantation of neuronsabruptly increased the latency of moving into the dark compartmentof the chamber in the conditioned avoidance response test.<br /><strong>Conclusions.</strong>The pilot experiment using the HD model showedthat neurotransplantation using iPSC derivatives recovers the reducedmotor activity in rats and improves memory trace keeping,which contributes to correction of motor and cognitive disordersinduced by 3-NPA neurotoxin.</p>Hungtington’s disease3-nitropropionic acidbehavior and memory disordersstriatumneurotransplantationinduced pluripotent stem cellsболезнь Гентингтона3-нитропропионовая кислотанарушения поведения и памятистриатумнейротрансплантацияиндуцированные плюрипотентные стволовые клетки[The Huntington’s Disease Collaborative Research Group. 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