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Preconditioning of ischemic and hypoxic type was investigated as a method of protecting brain against acute ischemic injury. The preconditioning methods were applied to experimental rats 24 h before the time when local brain infarct was done by middle cerebral artery occlusion (MCAO). It was found that the ischemic and hypoxic preconditioning resulted in three general morphological changes: 1) the size of infarct zone was reduced by 2.2–3.8 times compared with rats that had not been treated with the preconditioning before MCAO; 2) the preconditioning treatment retained the number of living neurons in penumbra at the level of control rats, while without the preconditioning neuronal count in the penumbra after MCAO was 29% lower; 3) the number of glial cells in penumbra was increased after MCAO by 38% compared with the control level, and continued to increase under the preconditioning treatment up to 60%, that suggests an important role of neuroglia in neuroprotection. Selective blockers of ATP2dependant K+2channels (52hydroxydecanoate and glibenclamide) completely abolished the neuroprotective effects of the preconditioning.
Preconditioning as a method of neuroprotection in a model of brain infarct
- Authors: Khudoerkov R.M.1, Samojlenkova N.S.2, Gavrilova S.A.3, Pirogov Y.A.2, Koshelev V.B.2
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Affiliations:
- Research Center of Neurology
- M.V. Lomonosov Moscow State University
- Lomonosov Moscow State University
- Issue: Vol 3, No 2 (2009)
- Pages: 26-30
- Section: Original articles
- URL: https://www.annaly-nevrologii.com/journal/pathID/article/view/374
- DOI: https://doi.org/10.17816/psaic374
- ID: 374
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About the authors
Rudolf M. Khudoerkov
Research Center of Neurology
Author for correspondence.
Email: rolfbrain@yandex.ru
Russian Federation, Moscow
N. S. Samojlenkova
M.V. Lomonosov Moscow State University
Email: rolfbrain@yandex.ru
Russian Federation, Moscow
Svetlana A. Gavrilova
Lomonosov Moscow State University
Email: rolfbrain@yandex.ru
Russian Federation, Moscow
Yu. A. Pirogov
M.V. Lomonosov Moscow State University
Email: rolfbrain@yandex.ru
Russian Federation, Moscow
V. B. Koshelev
M.V. Lomonosov Moscow State University
Email: rolfbrain@yandex.ru
Russian Federation, Moscow
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